MluCI from New England Biolabs

Product Specs
ItemMluCI
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0538L
Quantity5000 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Integration site–dependent HIV-1 promoter activity shapes host chromatin conformationGenome ResearchJune 1, 2023Jack A. Collora, Ya-Chi Howith 500 rpm shaking in 200 µL restriction enzyme mix 2 (1× CutSmart 200 U MluCI; New England Biolabs R0538L) for 4 h at 37°C. An additional 200 U of restriction enzyme was added, and the incubation continued

(2) Characterization of antibiotic resistomes by reprogrammed bacteriophage-enabled functional metagenomics in clinical strainsNature MicrobiologyFebruary 9, 2023Gábor Apjok, Mónika Számel, Chryso Christodoulou, Viktória Seregi, Bálint Márk Vásárhelyi, Tamás Stirling, Bálint Eszenyi, Tóbiás Sári, Fanni Vidovics, Erika Nagrand, et al.(Sigma, NA2110-1KT). From each sample, 40 µg of extracted DNA was digested with MluCI enzyme (NEB, R0538L) (10 min, 37 °C), followed by inactivation (20 min, 85 °C). The quantity of the MluCI enzyme was varied

(3) Hi-TrAC reveals division of labor of transcription factors in organizing chromatin loopsNat CommunNovember 5, 2022Shuai Liu, Yaqiang Cao, Kairong Cui, Qingsong Tang, Keji ZhaoAMPure XP beads with 80 μL elution buffer and digested with the restriction enzymes 2 μL MluCI (NEB, R0538L) and 2 μL NlaIII (NEB, R0125L) in 100 μL reaction mixture at 37 °C for 30 min. The reaction mixture

(4) Genomic consequences of domestication of the Siamese fighting fishScience AdvancesMarch 11, 2022Young Mi Kwon et al.enzyme site for MluCI. We PCR-amplified the region and overnight digested the amplicons with MluCI (R0538L, NEB) (tables S3 and S4). The PCR fragments were then visualized on a 2.5% agarose gel (fig. S21). To

(5) Generation of gene-level resolution chromosome contact maps in bacteria and archaeaSTAR ProtocolsJune 18, 2021Charlotte Cockram, Agnès Thierry, Romain KoszulIndexes),Illumina,Cat# 20015949 HpaII,New England Biolabs,Cat# R0171M MluCI,New England Biolabs,Cat# R0538L "DNA Polymerase I, Large (Klenow) Fragment",New England Biolabs,Cat# M0210L 10× NEBuffer 2,New England Biolabs

(6) Trac-looping measures genome structure and chromatin accessibilityNature methodsSeptember 1, 2018Lai B, Tang Q, Jin W, Hu G, Wangsa D, Cui K, Stanton BZ, Ren G, Ding Y, Zhao M, et al.The eluates were split into 2 tubes and digested with 33 μl of Nla III (NEB, R0125L) and MluCI (NEB, R0538L), respectively, at 37°C for 3 hours. To each of the reactions were added 50μl Streptavidin C1 beads

(7) Partitioning the effects of isolation by distance, environment, and physical barriers on genomic divergence between parapatric threespine sticklebackEvolutionFebruary 1, 2017Jesse N. Weber, Gideon S. Bradburd, Yoel E. Stuart, William E. Stutz, Daniel I. Bolnickmicroplate reader (TECAN). We digested DNA with 1 Unit of both NlaIII (NEB Cat# R0125L) and MluCI (NEB Cat# R0538L), and used AMPure SPRI beads (Beckman Coulter) for all enzymatic cleanups. We quantified digested products,

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MluCI

MluCI from New England Biolabs

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New England Biolabs

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MluCI from New England Biolabs

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